生物化學(xué)翻譯求助11
FIG. 2.
HAX-1 can be cleaved by Omi protease in vitro. In vitro translated 35S-labeled HAX-1 protein was incubated at 37 °C without (lane 1) or with 500 ng of recombinant His-Omi134–458 for 2 h (lane 2) and6h(lane 3). The reactions were resolved on SDS-PAGE, and the gel was transferred on a PDVF membrane and exposed on X-OMAT-AR film.



HAX-1 Protein Level Decreases during Cell Death—To investigate whether the level of HAX-1 protein is regulated in mammalian cells during cell death, we treated HK-2 cells with various concentrations of cisplatin or H2O2. Fig. 3 shows the protein level of HAX-1 proportionally decreases as the concentration of cisplatin is raised from 30 to 70 µM. HAX-1 protein also decreased in cells treated with increasing concentration of H2O2.