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  • 氨基酸序列分析問題:已知一個七肽的組成為:gly,phe(2),tyr,met,asp,用胰凝乳蛋白酶水解

    氨基酸序列分析問題:已知一個七肽的組成為:gly,phe(2),tyr,met,asp,用胰凝乳蛋白酶水解
    已知一個七肽的組成為:gly,phe(2),tyr,met,asp,用胰凝乳蛋白酶水解后可得一個三肽和一個四肽,三肽組成為phe,tyr,asp.此7肽經(jīng)CNBR處理后,再經(jīng)陽離子交換樹脂層析并洗脫得到一個組分為arg,phe,gly的三肽.此七肽經(jīng)胰蛋白酶水解可得phe,用FDNB反應(yīng)后再水解得DNP-tyr.請寫出此肽序列.
    更正:七肽的組成為:gly,phe(2),tyr,met,asp,arg
    其他人氣:976 ℃時間:2020-10-01 09:39:58
    優(yōu)質(zhì)解答
    (2)是啥意思?2個the answer is : NH2-tyr-phe-asp-met-gly-arg-phe-COOH求詳細(xì)的解析!??!謝謝First,F(xiàn)DNB is a technique to determine the n-terminus of the peptide, so the N-terminus of this polypeptide is tyr.then, pancreatic protease hydrolyze the polypeptide immediately after the carboxylic group of the acidic residue, that is asp, in this case. Since tyr, phe, and asp are together , the first 3 residues are : NH2-tyr-phe-asp.then, the pancreatic tripsin cuts the polypeptide after the carboxylic group of arg, so, the last two residues are -arg-phe-COOH.then, we are left with met and gly. Since arg, phe, gly are together,we now can be sure that the fourth one is met, and the fifth one is gly,so the final sequence is NH2-tyr-phe-asp-met-gly-arg-phe-COOHpancreatic protease hydrolyze the polypeptide immediately after the carboxylic group of the acidic residue, that is phe,not asp.
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