英語翻譯
Preincubation of SLK cells with free cRGD peptide significantly
reduced the cytotoxic response of SLK cells to 16%
cRGD-DOXO-SPIO micelles (PG:67.1±5.0%),supporting
the X-dependent growth inhibition.In the current micelle
design,encapsulation of DOXO and SPIO nanoparticles
inside the hydrophobic micelle cores has the advantages of
avoiding potential exposure of hydrophobic SPIO surfaces
and adsorption of blood proteins.In addition,polymer chain
entanglement led to a very low critical micelle concentration
(CMC) at 12 ug/mL.All of the experiments in the current
study were performed at concentrations much higher than
the CMC to ensure the integrity of micelles.“Stealth”
micelles with similar PEG-PLA copolymer compositions
have demonstrated significantly increased in vivo half-lives
of drugs (e.g.,paclitaxel) to 11 h in cancer patients.The
increased in vivo stability should enhance the effectiveness
of these cRGD-functionalized micelles for active targeting
to tumor vasculature.Current work is in progress to evaluate
the in vivo efficacy of the cRGD-DOXO-SPIO micelles in
an animal tumor model.