5ml 7.5%分離膠：
H2O 2.35ml
30%丙烯酰胺溶液 1.25ml
1.5M Tris(pH8.8) 1.3ml
10%SDS 50ul
10%APS 50ul
TEMED 4ul
5ml 4%濃縮膠：
H2O 3ml
30%丙烯酰胺溶液 670ul
0.5M Tris(pH6.8) 1.25ml
10%SDS 50ul
10%APS 50ul
TEMED 4ul