PCR is typically used to amplify DNA that lies between two known sequences.
PCR is typically used to amplify DNA that lies between two known sequences.
Suppose that you want to explore DNA on both sides of a single known sequence. Devise
a variation of the usual PCR protocol that would enable you to amplify entirely new genomic terrain.
怎么理解這道題目是什么意思?
Suppose that you want to explore DNA on both sides of a single known sequence. Devise
a variation of the usual PCR protocol that would enable you to amplify entirely new genomic terrain.
怎么理解這道題目是什么意思?
英語人氣:270 ℃時間:2020-06-12 00:46:37
優(yōu)質(zhì)解答
是不是說:原本pcr是用來擴增兩個primer之間的序列的,現(xiàn)在要explore一段已知序列的兩邊,所以只要把那段序列的兩個方向先分成兩段,然后第一遍擴增后就可以得到最端的序列,然后再進行usual pcr...能否再說的詳細點呢?謝謝啊嘞嘞。。。這個。。。我覺得。就是傳說中的5‘extension、、、就是在中間序列已知的情況下。。利用這個dna 2條連都可以用來pcr的特點,找到其中一個的5’端(就是另一條的3‘端),然后就可以完全復制這個序列了。。。特征主要是在已知一條3’時。。。pcr的時間不要太長,創(chuàng)造出primer、、、、吶。。。大概就是這個意思。。。。
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