取決于你要的醋酸濃度和pH及甲基綠的濃度,你查文獻.總之先配醋酸鈉緩沖液,再放甲基綠.
GENERAL recipe:
0.1M Sodium Acetate Buffer,pH4.2:
Sodium acetate,trihydrate (MW 136.1) ------ 1.36 g
Distilled water ----------------------------------- 100 ml
Mix to dissolve and adjust pH to 4.2 using concentrated glacial acetic acid
Methyl Green Solution (0.5%)
Methyl green (ethyl violet free from Sigma) ---- 0.5 g
0.1M Sodium acetate buffer,pH4.2 ------------- 100 ml
Mix to dissolve.